Naringenin promotes SDF-1/CXCR4 signaling pathway in BMSCs osteogenic differentiation

Introduction. Naringenin, a dihydro-flavonoid compound that shows chemotactic activity, may have good application prospect in repairing bone tissue, but its specific mechanism regeneration, especially the osteogenic differentiation of stem cells, needs for further study. The aim this study was to investigate effect naringenin on and roles C-X-C chemokine receptor type 4/stromal cell-derived factor 1 (SDF-1/CXCR4) signal pathway marrow-derived mesenchymal cells (BMSCs). Material methods. BMSCs were harvested from femurs tibias 4-to-6-week-old male Sprague-Dawley rats. Cell Counting kit-8 assay used determine cytotoxicity naringenin. Alkaline phosphatase (ALP) activity measured cell’s precipitates alizarin-red staining performed capacity BMSCs. Real-time polymerase chain reaction, enzyme-linked immunosorbent western blotting adopted expression genes proteins. Results. cellular morphology spindle-shaped, arranged radial whorled patterns. flow cytometric analysis confirmed presence characteristic surface proteins Different concentrations (0–200 μg/ml) no influence viability proliferation rate highest ALP found at culture day 7 9 when concentration 75 100 μg/ml. Positive red or dark stained with mineralized nodules can be observed 14. ALP, Runt-related transcription 2, CXCR4 SDF-1a gene protein levels naringenin-treated significantly higher than those control cells. Moreover, AMD3100, an inhibitor CXCR4, suppressed studied Conclusions. Naringenin does not show toxic promotes via SDF-1/CXCR4 signaling pathway. A better understanding mechanisms action would helpful developing therapeutic strategies improve regeneration after injuries.